Fellow: Javier Jaldin-Fincati, PhD
Institution: The Hospital for Sick Children, Toronto, Canada
Principal Investigator: Amira Klip, PhD
LF Funding History: HRiA/LE&RN/FDRS Postdoctoral Fellowship
Hypothesis: We hypothesize that the products of adipocyte metabolism may affect the normal function of lymphatic microvascular endothelial cells.
Collaborative Opportunities: Javier and Amira look to explore collaborations with leading researchers in adipose cell differentiation and lymphatic system development.
Project: Communication Between Adipose and Lymphatic Microvascular Endothelial Cells
We hypothesize that products of adipocyte metabolism may affect the normal function of lymphatic microvascular endothelial cells (L-MEC), contributing to lipedema. We think that if lymphatic capillaries do not drain 'toxic materials' out of adipose tissue, such as fatty acids, an inflammatory state is generated. If we add to this an insufficient removal of excess insulin, a vicious cycle may ensue of insulin resistance in fat cells and inflammation in the tissue that will further damage lymphatic capillaries.
In this context, it is essential to investigate the effect of increased levels of insulin and fatty acids on the metabolism and viability of L-MEC. To address this, we will use L-MEC from human dermal tissue and cultivate them under normal and pathological levels of adipocyte metabolites. This will lead to a better understanding of how these cells respond to healthy and sick environments created by adipose tissue. Subsequent work will examine the influence of pro-inflammatory products and cells on the integrity and survival of lymphatic cells in culture.